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PDZ domain-containing 8 (PDZD8) knockdown inhibits palmitic acid (PA)-induced insulin 1 (INS-1) cell death. (A, B) T he apoptosis of INS-1 cells was detected using the flow cytometry. (C) The cytotoxicity was detected by lactate <t>dehydrogenase</t> <t>(LDH)</t> kit. (D) The expression of mitochondrial apoptosis-related proteins was detected by Western blotting. PE-H, phycoerythrin-H; FITC-H, fluorescein isothiocyanate-H; siPDZD8, siRNA PDZ domain-containing 8; Bcl-2, B cell lymphoma 2; Bax, BCL2 associated X, apoptosis regulator; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. a P <0.05 vs. control (Con) group, b P <0.05 vs. PA+shRNA negative control (shNC) group.
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PDZ domain-containing 8 (PDZD8) knockdown inhibits palmitic acid (PA)-induced insulin 1 (INS-1) cell death. (A, B) T he apoptosis of INS-1 cells was detected using the flow cytometry. (C) The cytotoxicity was detected by lactate dehydrogenase (LDH) kit. (D) The expression of mitochondrial apoptosis-related proteins was detected by Western blotting. PE-H, phycoerythrin-H; FITC-H, fluorescein isothiocyanate-H; siPDZD8, siRNA PDZ domain-containing 8; Bcl-2, B cell lymphoma 2; Bax, BCL2 associated X, apoptosis regulator; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. a P <0.05 vs. control (Con) group, b P <0.05 vs. PA+shRNA negative control (shNC) group.

Journal: Diabetes & Metabolism Journal

Article Title: PDZD8 Augments Endoplasmic Reticulum-Mitochondria Contact and Regulates Ca 2+ Dynamics and Cypd Expression to Induce Pancreatic β-Cell Death during Diabetes

doi: 10.4093/dmj.2023.0275

Figure Lengend Snippet: PDZ domain-containing 8 (PDZD8) knockdown inhibits palmitic acid (PA)-induced insulin 1 (INS-1) cell death. (A, B) T he apoptosis of INS-1 cells was detected using the flow cytometry. (C) The cytotoxicity was detected by lactate dehydrogenase (LDH) kit. (D) The expression of mitochondrial apoptosis-related proteins was detected by Western blotting. PE-H, phycoerythrin-H; FITC-H, fluorescein isothiocyanate-H; siPDZD8, siRNA PDZ domain-containing 8; Bcl-2, B cell lymphoma 2; Bax, BCL2 associated X, apoptosis regulator; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. a P <0.05 vs. control (Con) group, b P <0.05 vs. PA+shRNA negative control (shNC) group.

Article Snippet: INS-1 cells were cultured in 96-well plates (5×10 3 cells/well) for 24 hours and then incubated with lactate dehydrogenase (LDH) release reagent (C0016; Beyotime) at 37°C for 1 hour.

Techniques: Knockdown, Flow Cytometry, Expressing, Western Blot, Control, shRNA, Negative Control